Figure 3.

Effects of paclitaxel (PTX) on PC14PE6 and NCI‐H441 cell lines in vitro. (A) PC14PE6/EF‐Luc and (B) NCI‐H441/EF‐Luc cells were seeded into 24‐well plates and the following day various concentrations of PTX (as indicated) were added to the culture medium and incubated for another 48 h under normoxic or hypoxic conditions. The percentage of dead cells with degraded genomic DNA was analyzed using fluorescence‐activated cell sorting (FACS) after staining genomic DNA with propidium iodide. Each experiment was done in triplicate and the average dead cell percentage in each group is shown. Experiments were repeated more than three times and representative data is shown. Error bars indicate SEM. (C) Representative FACS data of each group.