Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Western blot analysis of hypoxia inducible factor (HIF)‐1α protein expression in the PC14PE6 and NCI‐H441 cells. The cells were cultured under normoxic (N) and hypoxic (H) conditions for 24 h and then cells were harvested and lyzed with western lysis buffer under normoxic or hypoxic conditions. The lysates were subjected to an immunoblotting analysis with anti‐HIF‐1α and anti‐glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) antibodies as described in Materials and Methods.
