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. 2009 Feb 17;100(4):737–746. doi: 10.1111/j.1349-7006.2009.01083.x

Figure 4.

Figure 4

Both membrane and mitochondrial caspase pathways were activated by dehydroxymethylepoxyquinomicin (DHMEQ) treatment of primary effusion lymphoma (PEL) cells. (A) Cleaved products of caspases were detected after DHMEQ treatment (10 µg/mL) for 6 h. Caspase 3, responsible for the common pathway, caspase 8, as an indicator for the membrane pathway, and caspase 9 as an indicator for the mitochondrial pathway. Jurkat cell line treated with etoposide 50 µM for 4 h served as a control. DNAs were stained by Hoechst 33258 (blue fluorescence). Green fluorescence indicated cleaved products of caspases bound to FLICA peptides. (B) Immunoblot analysis of caspase cleavage. TY1 and BC1 cells were treated with 10 µg/mL of DHMEQ for 6 h. Samples of 30 µg of whole cell lysates were examined. Positions of cleaved forms of caspase 3 and 9 are indicated on the left of the upper two panels (arrows). In the third panel, uncleaved and cleaved forms of caspase 8 are indicated on the left (arrowhead: uncleaved). An immunoblot of α‐tubulin served as a control (bottom panel).