Table 2.
Quantitative results of staining primary effusion lymphoma (PEL) cell lines with Annexin V and propidium iodide (PI)
| DHMEQ | BCBL1 (%) | TY1 (%) | BC1 (%) | |
|---|---|---|---|---|
| UL | (–) | 0.71 ± 0.14 | 1.96 ± 1.07 | 0.55 ± 0.09 |
| (+) | 7.13 ± 3.82 | 6.06 ± 0.50 | 5.04 ± 1.60 | |
| UR | (–) | 1.94 ± 0.57 | 2.84 ± 1.92 | 1.96 ± 1.02 |
| (+) | 45.92 ± 17.04 | 37.89 ± 15.36 | 38.53 ± 20.95 | |
| LL | (–) | 95.37 ± 0.29 | 90.91 ± 4.07 | 93.10 ± 2.13 |
| (+) | 34.70 ± 11.14 | 33.62 ± 0.82 | 43.58 ± 12.14 | |
| LR | (–) | 1.96 ± 0.45 | 4.28 ± 1.61 | 4.37 ± 2.20 |
| (+) | 12.34 ± 2.97 | 22.41 ± 14.32 | 12.83 ± 9.69 |
PEL cells incubated with or without dehydroxymethylepoxyquinomicin (DHMEQ) (10 µg/mL) for 24 h and stained with Annexing V and PI. Then, analyzed with flow cytometry. Upper left (UL), upper right (UR), lower left (LL) and lower right (LR) quadrants are responsible for PI positive only, PI and AnnexinV positive, PI and Annexin V negative, Annexin V positive only cell populations, respectively. Results are mean value ± SD of 3 triplicate independent experiments.