Table 1.
50% inhibitory concentration (IC50) values of coenzyme Q (CoQ) for the activities of various DNA polymerases and other DNA metabolic enzymes
| Enzyme | IC50 values of coenzyme Q (µM) | ||||
|---|---|---|---|---|---|
| CoQ1 | CoQ2 | CoQ4 | CoQ6 | CoQ10 | |
| Mammalian DNA polymerases | |||||
| Calf DNA polymerase α | >200 | >200 | >200 | >200 | >200 |
| Rat DNA polymerase β | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase γ | |||||
| *DNA polymerase activity | 12.2 | 45.6 | 145 | >200 | >200 |
| **Reverse transcriptase activity | 14.5 | 49.0 | 151 | >200 | >200 |
| Human DNA polymerase δ | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase ɛ | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase η | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase ι | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase κ | >200 | >200 | >200 | >200 | >200 |
| Human DNA polymerase λ | >200 | >200 | >200 | >200 | >200 |
| Fish DNA polymerases | |||||
| Cherry salmon DNA polymerase α | >200 | >200 | >200 | >200 | >200 |
| Cherry salmon DNA polymerase δ | >200 | >200 | >200 | >200 | >200 |
| Insect DNA polymerases | |||||
| Fruit fly DNA polymerase α | >200 | >200 | >200 | >200 | >200 |
| Fruit fly DNA polymerase δ | >200 | >200 | >200 | >200 | >200 |
| Fruit fly DNA polymerase ɛ | >200 | >200 | >200 | >200 | >200 |
| Plant DNA polymerases | |||||
| Cauliflower DNA polymerase I (α‐like) | >200 | >200 | >200 | >200 | >200 |
| Cauliflower DNA polymerase II (β‐like) | >200 | >200 | >200 | >200 | >200 |
| Rice DNA polymerase λ | >200 | >200 | >200 | >200 | >200 |
| Procaryotic DNA polymerases | |||||
| E. coli DNA polymerase I (Klenow fragment) | >200 | >200 | >200 | >200 | >200 |
| Taq DNA polymerase | >200 | >200 | >200 | >200 | >200 |
| T4 DNA polymerase | >200 | >200 | >200 | >200 | >200 |
| Other DNA metabolic enzymes | |||||
| Calf primase of DNA polymerase α | >200 | >200 | >200 | >200 | >200 |
| Calf terminal deoxynucleotidyltransferase | >200 | >200 | >200 | >200 | >200 |
| HIV‐1 reverse transcriptase | >200 | >200 | >200 | >200 | >200 |
| T7 RNA polymerase | >200 | >200 | >200 | >200 | >200 |
| Human DNA topoisomerase I | >200 | >200 | >200 | >200 | >200 |
| Human DNA topoisomerase II | 12.5 | >55.0 | >200 | >200 | >200 |
| T4 polynucleotide kinase | >200 | >200 | >200 | >200 | >200 |
| Bovine deoxyribonuclease I | ≥200 | ≥200 | ≥200 | ≥200 | ≥200 |
These compounds were incubated with each enzyme (0.05 units). The enzymatic activity was measured as described in Materials and Methods. The enzymatic activity in the absence of the compounds was taken as 100%. *The template‐primer is poly(dA)/oligo(dT)12−18, A/T = 2/1. **The template‐primer is poly(rA)/oligo(dT)12−18, A/T = 2/1. HIV‐1, human immunodeficiency virus type‐1.