Figure 4.

Image of two‐dimensional (2‐D) gel stained with Coomassie Blue R‐250. B16F1 cells were treated with methylene‐blue‐mediated photodynamic therapy (MB‐PDT) (20 µM). 6 h after irradiation, the treated cells were lyzed in 4% CHAPS buffer and assayed for protein concentration. 1 mg of total protein was prepared for two‐dimensional gel separation. (a) Representative example of gel image from MB‐PDT treated group showing the entire pI range 3–10 (non‐linear) and molecular masses of 10–100 kDa. (b) Differential protein expression analysis with zoomed images of selected gel areas. The number in each zoomed image presents the reduction fold‐change compared with MB‐PDT. The selected subset of proteins, as listed in Table 1, is shown.