Figure 6.

Methylene‐blue‐mediated photodynamic therapy (MB‐PDT) induces apoptotic cell death in vivo. Samples were gained from treated mice as described above. (a) In situ detection of apoptotic cells by fluorescence microscopy using the terminal 2′‐deoxyuridine‐5′‐triphosphate (dUTP) nick end labeling (TUNEL) assay. Bright‐field image (up), TUNEL reaction fluorescence (middle) and merge of both (down) sections from melanomas treated by MB‐PDT as indicated. (b) Immunohistochemical staining of melanoma sections using anti‐cleaved caspase‐3 antibody. Shown is 1 representative experiment of 3. Original magnifications: up ×100; down ×200.