Figure 1.

 Lymphatic endothelial cells (LECs) induced by LNMTca8113 cells and Tca8113 cells displayed different biologic behaviors. (a) LEC proliferation. LEC proliferation was determined by (³H)thymidine incorporation into the cell DNA. In contrast to Tca8113‐stimulated LECs (TLECs), the proliferation activity of LNMTca8113‐stimulated LECs (LNMLECs) increased moderately (P = 0.017). (b) Lymphangiogenesis. By the measurement of the length of tubules, it was confirmed that lymphangiogenesis could be enhanced by addition of LNMTca8113 conditioned medium (CM) (P = 0.012). (c) LEC apoptosis. The number of apoptotic LECs was determined by Annexin V‐PI staining using a FACSCalibur. The results showed that LNMLECs and TLECs were equal in resisting endothelial cell growth supplement (ECGS) starvation‐induced apoptosis (P = 0.701).