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. 2008 Mar 28;99(4):810–815. doi: 10.1111/j.1349-7006.2008.00741.x

Figure 1.

Figure 1

Expression of various DNA double‐strand breaks repair proteins after BRCA2 siRNA transfection. Cells were transfected with various concentrations of BRCA2 siRNA for 48 h and compared with the non‐treated and the mock‐treated control samples. (a) BRCA2 and Rad51 protein expressions were detected by Western blot (upper graph). (b) Cells were transfected with mock‐treated control, negative siRNA or 20 nM BRCA2 siRNA for 48 h, and the levels of BRCA2, DNA‐PKcs, Ku70, Ku80, expressions were determined by Western blots. (c) The expression of BRCA2 messenger RNA was examined by reverse transcription‐polymerase chain reaction. Cells were transfected with 20 nM BRCA2 siRNA for 48 h. Data represent the mean and the standard deviation from three independent experiments. *, P < 0.05 versus control or mock‐transfected cells (Student's t‐test).