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. 2009 Nov 18;101(3):774–781. doi: 10.1111/j.1349-7006.2009.01446.x

Figure 1.

Figure 1

 Inhibitory effect of JTE‐607 on spontaneous and lipopolysaccharide (LPS)‐stimulated cytokine production from cultured acute myelogenous leukemia (AML) cells. (A) HL‐60 and U‐937 cells were exposed to JTE‐607 (0.01 to 1 μm) for 2 h. Interleukin (IL)‐6, IL‐8, and vascular endothelial growth factor (VEGF) mRNA levels were determined by quantitative RT‐PCR, and the values normalized to GAPDH mRNA levels are shown. Each bar represents the mean ± SEM of triplicate measurements. (B) HL‐60 and U‐937 cells were exposed to JTE‐607 (0.1 and 1 μm) for 24 h. IL‐8 protein levels in the culture supernatants were measured by ELISA. Each bar represents the mean ± SEM of triplicate measurements. (C,D) THP‐1 and U‐937 cells were stimulated with phorbol myristate acetate (PMA) (C, 10 ng/mL) or LPS (D, 10 μg/mL) in the presence of JTE‐607 at indicated concentrations for 24 h. VEGF (C) and IL‐8 (D) protein levels in the culture supernatants were measured by ELISA. Each bar represents the mean of duplicate measurements.