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. 2009 Nov 3;101(2):523–529. doi: 10.1111/j.1349-7006.2009.01424.x

Figure 1.

Figure 1

 Quantification and validation of protein over‐expression in pooled colorectal cancer (CRC) samples. (a) The expression level of transgelin‐2 (TAGLN2) was quantified to be four‐fold higher by calculating the areas under the monoisotopic peaks of the heavy isotopic (D3) versus light isotopic (D0) peptides. (b) Western blot analysis confirmed the over‐expression of TAGLN2 in tumor samples. The results of the Western blot for solute carrier family 12 member 2 (SLC12A2) and ras‐related protein Rab‐10 (RAB10) were also consistent with the D0/D3 acetylation analysis.