Figure 1.

 Regulatory T cells (Tregs) were increased in draining lymph node (DLN), but not distal LN or spleen, in squamous cell carcinoma (SCC)‐bearing host mice. (A) CMC‐1 (2 × 10⁶; representative SCC) was inoculated intradermally. The numbers of CD4 ⁺ Foxp3 ⁺ T cells in tumor‐side axillary DLN (filled circle), contralateral axillary LN (distal LN; open circle), and spleen (filled triangle) was analyzed 0, 4, 8, 12, and 16 days after CMC‐1 inoculation. The data shown are mean ± SD of three mice in each experimental group. *P < 0.05 DLN versus distal LN. Similar results were obtained in two separate experiments. (B) Tregs were detected in LN and spleen of normal mice, and DLN, distal LN, and spleen of tumor‐bearing mice by immunohistochemical analysis. Representative data are shown. Green, Foxp3; red, CD4. Magnification, ×200.