Figure 2.

 The number of regulatory T cells (Tregs) in draining lymph node (DLN) increases in 3‐methylcholanthrene (MCA)‐induced squamous cell carcinoma (SCC)‐bearing mice, but not in sarcoma‐bearing mice. (A) BALB/c mice were inoculated intradermally with primary sarcoma lines (CMS‐G2 and CMS‐G4; 2 × 10⁶) and primary SCC lines (CMC‐1 and CMC‐9; 2 × 10⁶). DLN of the tumor‐bearing mice were immunostained with anti‐CD4 mAb and anti‐Foxp3 mAb. Distributions of CD4 ⁺ Foxp3 ⁺ Tregs in the tumor DLN were evaluated by immunohistochemical observation. Green, Foxp3; red, CD4. Magnification, ×200. (B) The numbers of Foxp3 ⁺ Tregs of the sections were counted. The data shown are mean ± SD of five fields of each DLN. *P < 0.02 sarcoma versus SCC.