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. 2010 Jun 9;101(9):2011–2018. doi: 10.1111/j.1349-7006.2010.01640.x

Figure 5.

Figure 5

 Detection of heregulin (HRG) in the cultured hepatocytes and liver with LS‐LM6 metastasis, and suppression of LS‐LM6 migration after removal of HRG from hepatocyte‐conditioned medium (HCM). (a) Reverse transcription (RT)‐PCR of the cultured hepatocytes demonstrates HRG (m‐HRG) at the expected 539‐bp band (GAPDH as a control). Western blot of concentrated HCM shows the presence of HRG with the maximum signal on day 3. (b) The enhanced LS‐LM6 migration is significantly attenuated by removal of HRG from the HCM (*P < 0.05, Student’s t‐test, lower panel). (c) In the liver with LS‐LM6 metastasis, some hepatocytes are positive for HRG immunohistochemistry whereas no positive staining is detected in the normal control liver nor metastatic nodules of LS‐LM6. Ca, carcinoma. (d) Reverse transcription (RT)‐PCR demonstrates HRG (h‐HRG) at the expected 236‐bp band in LS174T and LS‐LM6. Western blot shows a small amount of HRG in the concentrated conditioned medium of LS174T and LS‐LM6. The conditioned medium of LS‐LM6 does not induce the migration of LS‐LM6 (*P < 0.05, two‐way anova).