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. 2008 Apr 14;99(6):1180–1187. doi: 10.1111/j.1349-7006.2008.00804.x

Figure 4.

Figure 4

Epidermal growth factor receptor (EGFR) phosphorylation induced by EGF was more apparent in the cells with CA‐repeat lengths in the longer/shorter alleles of 16/15 and 8/7 compared to cells with CA‐repeat lengths of 20/19. Normal human bronchial epithelial (HBE) cells were first incubated for 24 h in medium without EGF and bovine pituitary extract, then treated with 100 ng/mL EGF for the indicated periods. The numbers of CA‐repeats were 20/19 in patients 10, 9, 8, 16/15 in patient 1, and 8/7 in patient 12. Western blot analysis was conducted on whole cell lysates (50 µg) (a). The relative ratio of EGFR phosphorylation was determined as the intensity of EGFR phosphorylation divided with that of EGFR (b).