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. 2005 Dec 9;96(12):866–872. doi: 10.1111/j.1349-7006.2005.00126.x

Figure 2.

Figure 2

Detection of mutant breast cancer resistance proteins (BCRP), containing serine substitutions, by western blot analysis. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) was carried out (a) under reducing conditions with heating at 70°C for 10 min, or (b) under non‐reducing conditions with heating at 20°C for 5 min. α‐Tubulin expression was analyzed as a loading control. (c) Western blot analysis of three BCRP mutants with a long exposure showing weak band intensities under non‐reducing conditions. Cellular protein (20 µg/lane) was separated by 5–20% SDS‐PAGE and then transferred onto nitrocellulose membranes. BCRP was detected using mouse anti‐BCRP monoclonal antibody (BXP‐21).