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. 2005 Dec 9;96(12):866–872. doi: 10.1111/j.1349-7006.2005.00126.x

Figure 5.

Figure 5

SN‐38 sensitivity assay of mutant breast cancer resistance protein (BCRP)‐transfected PA317 cells. Drug sensitivity to SN‐38 was examined by cell growth inhibition assays as described in Materials and Methods. All data are representative of the mean values ± SD from triplicate determinations. (a) SN‐38 sensitivity of the indicated mutant BCRP transfectants, which expressed similar levels of BCRP protein to PA/WT. (b) SN‐38 sensitivity of four mutant BCRP transfectants, which expressed lower levels of BCRP protein than PA/WT. PA/C43S, PA/C55S, PA/C284S and PA/C603S transfectants acquired similar or somewhat lower degrees of SN‐38 resistance than PA/WT cells. (c) SN‐38 sensitivity of mutants that expressed very small amounts of monomer BCRP. PA/C592S and PA/C608S showed decreased drug resistance and the sensitivity of PA/C438S cells was similar to that of the parental PA317 cells.