Figure 1.

 Hypoxia‐inducible factor‐1α (HIF‐1α) is affected by nitric oxide (NO) reduction in hypoxic lung cancer cells. Expression of HIF‐1α was reduced with combined treatment in H3255 (*P = 0.01) and A549 (**P = 0.08) cells (a,b). Inducible nitric oxide synthases (iNOS) level was confirmed following the treatment with N ^G‐monomethyl‐l‐arginine, monoacetate (LNMMA) in both cell lines (P = 0.03 and P = 0.06, in A549 and H3255, respectively) (c,d). Consequently, more than 98% reduction in nitrate concentration in the culture media was detected (e). Values are expressed as the means ± SEM of three independent sets of data.