Skip to main content
. 2007 Jan 10;98(3):424–430. doi: 10.1111/j.1349-7006.2006.00382.x

Figure 5.

Figure 5

In vitro hyperthermia induced by 4‐S‐cysteaminylphenol (4‐S‐CAP)/magnetite cationic liposomes (MCL). (a) Uptake of magnetite nanoparticles into B16 cells at 24 h after addition of MCL or 4‐S‐CAP/MCL. Percentage magnetite uptake was evaluated. Data and bars are mean ± SD of three independent experiments (*P < 0.05). (b) Temperature increase in cell pellets treated with 4‐S‐CAP/MCL during alternating magnetic field (AMF) irradiation. B16 cells with (•) or without (○) 4‐S‐CAP/MCL were irradiated with AMF for 30 min. Data and bars are mean ± SD of three independent experiments (*P < 0.05). (c) In vitro antiproliferation effects of 4‐S‐CAP/MCL after AMF irradiation. After the AMF irradiation, cells were reseeded and the number of viable cells was measured on the indicated day by the trypan blue exclusion method using a hemocytometer. (○) Control B16 cells; (•) B16 cells with 4‐S‐CAP/MCL; and (▵) B16 cells treated with 4‐S‐CAP/MCL and AMF irradiation. Data and bars are mean ± SD of three independent experiments. *P < 0.05, significantly different from control group (non‐treated B16 cells); **P < 0.05, significantly different from 4‐S‐CAP/MCL group (4‐S‐CAP/MCL alone).