Figure 3.

 Knock down of RPS4X with specific siRNA molecules in (a,c) MCF7 and (b,d) MDA‐MB‐231 cells. Cells were transfected with the indicated siRNA molecules and, 96 h later, proteins were extracted for western blot analyses. (a,b) Representative blots following transfection of MCF7 (a) and MDA‐MB‐231 (b) cells with siRNAs against RPS4X. siControl, non‐specific siRNA molecules. In every blot, β‐actin and KU86 were used as loading controls. (c,d) Effect of RPS4X depletions on bromodeoxyuridine (BrdU) incorporation in MCF7 (c) and MDA‐MB‐231 (d) cells transfected with the indicated siRNA sequences, as determined by ELISA. Cells were transfected with the indicated siRNA sequences for 72 h and then incubated with medium containing BrdU for an additional 24 h before the ELISA. Data are the mean ± SEM (n = 4). *P < 0.03 (unpaired Student’s t‐test).