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. 2011 May 5;102(7):1410–1417. doi: 10.1111/j.1349-7006.2011.01948.x

Figure 6.

Figure 6

 Resistance of Y box‐binding protein 1 (YB‐1)‐overexpressing cells to cisplatin after RPS4X depletion. (a) MDA‐MB‐231 cells were transfected with either siControl (non‐specific siRNA molecules), siRPS4X, empty or YB‐1 expression vectors and treated with the indicated cisplatin concentrations. Experiments were performed in duplicate. Cells were treated with cisplatin for 18 h, after which they were plated (1000 cells per plate) in 100‐mm Petri dishes and cultured for 10 days without drug to allow colony formation. The y‐axis is in logarithmic scale. (b) Tandem affinity purification (TAP) or TAP‐YB‐1 MCF7 cells were transfected with siControl or siRPS4X molecules as indicated and treated with cisplatin for 18 h. The percentage of live cells was counted with a hemocytometer the next day. Experiments were performed in triplicate. Data are the mean ± SEM.