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. 2007 Jan 31;98(4):512–520. doi: 10.1111/j.1349-7006.2007.00419.x

Figure 1.

Figure 1

Expression analysis of caveolin‐1 and establishment of stable transfectants. a, Immunoblotting to examine the expression levels of caveolin‐1 in SK‐MEL‐28 and N1, a mutant line of SK‐MEL‐28, was performed using a rabbit anti‐caveolin‐1 antibody. b, After transfection of SK‐MEL‐28 cells with a caveolin‐1 synthase gene expression vector (pCMV‐Tag 3/caveolin‐1), or pCMV‐Tag 3, two transfectant lines (Cav12, Cav35) and two vector controls (V1 and V2) were established. Total cell lysates were subjected to immunoblotting with anticaveolin‐1 (upper panel) or antic‐myc (middle panel) antibodies. The location of the fusion protein is indicated by arrows.