Figure 2.

Effects of caveolin‐1 expression on cell proliferation, motility and invasion. a, Analysis of cell proliferation. Cells (2 × 10³) were seeded onto 96‐well plates. On days 1, 3, 6, and 9 of culture, the MTT assay was performed. Data are presented as relative absorbance, with absorbance on day 1 set as 1.0. *: P < 0.05, **: P < 0.001. b and c, Cell motility of the transfectants was analyzed using wound‐healing scratching motility assays. B, Photomicrographs of the scratched regions of vector control (V1) and Cav‐1⁺ transfectant (Cav12) cultures at times 0, 12, and 24 h. C, Results of the motility assay. Wound areas are presented as a percentage of the initial wound size (100%). The time course of the wound areas at times 0, 6, 12, 18, and 24 h is shown. Mean values (n = 12) were plotted for individual points. *: P < 0.05. d, Result of invasion assay. No significant difference in invasion activity, as analyzed by using the Boyden chamber, was found between control and Cav‐1⁺ transfectant cells.