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. 2007 Jan 31;98(4):512–520. doi: 10.1111/j.1349-7006.2007.00419.x

Figure 3.

Figure 3

Reduced phosphorylation of p130Cas and paxillin after fetal calf serum (FCS) treatment of transfectant cells. a, Time course of the phosphorylation levels of p130Cas and paxillin in vector control (V1 and V2) and Cav‐1+ (Cav12 and Cav35) cells. Cells (2 × 105) were treated with FCS after serum starvation for 12 h, and the phosphorylation levels of p130Cas and paxillin were observed up to 60 min after addition of FCS. Immunoblotting was carried out using PY20. b, Relative intensities of bands in a were plotted. c, Identification of bands at 130 kDa and 68 kDa as p130Cas and paxillin, respectively. Immunoprecipitation/immunoblotting (IP/IB) was performed to identify the two tyrosine‐phosphorylated components using PY20 and antip130Cas (a), and PY20 and antipaxillin antibodies (b) in vector control (V1) and Cav‐1+ cells (Cav35). Immunoprecipitates with PY20 (PY20 IP) and those with antip130Cas (p130Cas IP) together with total lysate were immunoblotted using PY20 (Ca, Cb, left) or anti‐p130Cas (Ca, Cb, right). d, Immunoprecipitates with PY20 or antipaxillin antibody were immunoblotted as performed for p130Cas in a to identify the 68 kDa band as paxillin (Da, Db).