Figure 4.

Alteration in the floatation pattern of GD3 in sucrose density gradient fractionation. Fractionation patterns for control and Cav‐1⁺ cells are shown. Triton X‐100 cell extracts were fractionated by sucrose density gradient ultracentrifugation, and each fraction was used for immunoblotting. Reagents used for the detection of individual molecules are described in the Materials and Methods. GD3 was detected with mAbR24. a, Results for the vector control cells (V1 and V2). b, Results for the Cav‐1⁺ cells (Cav12 and Cav35). Note that GD3 was widely distributed inside and outside of the GEM/rafts in the Cav‐1⁺ cells.