Skip to main content
. 2007 Jan 31;98(4):512–520. doi: 10.1111/j.1349-7006.2007.00419.x

Figure 7.

Figure 7

TLC and mass spectrometry analysis of GD3 in the GEM/raft and non‐GEM/raft fractions. a, Gangliosides were extracted from GEM/raft (2 and 3) and non‐GEM/raft (5–7 and 8–10) fractions from vector control (V1) and Cav‐1+ (Cav12) cells, then separated by TLC. The solvent used was chloroform/methanol/2.5 N NH4Cl (55:45:10). Resorcinol spray was used for the detection of bands. B, Molecular species of GD3 were analyzed by ESI‐MS using a Q‐Tof microTM quadrupole time‐of‐flight hybrid mass spectrometer (Micromass, Manchester, UK) with an Ultimate HPLC system as described in the Materials and Methods. The results of samples from GEM/raft (2 and 3) and non‐GEM/raft (5–7) fractions are shown. Peaks at 1442.90–1442.98 (m/z) were assigned d18:1–16:0, those at 1555.07–1555.11 were d18:1–24:0, and those at 1553.08–1553.11 were 18:1–24:1.