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. 2010 Apr 28;101(8):1813–1821. doi: 10.1111/j.1349-7006.2010.01605.x

Figure 3.

Figure 3

 Expression of mutant breast cancer resistance protein (BCRP) in selected transfectant clones. Breast cancer resistance protein (BCRP) expression in a mixed population of cells (‐mix) and in two transfectant clones with relatively high BCRP expression (‐cl.X) was examined. PA/WT‐mix was used as a control cell line. Western blot analysis was performed under reducing (+SH) and non‐reducing (−SH) conditions. GAPDH expression was analyzed as an internal control of protein loading in reducing conditions. (a) PA/WT cells; (b) PA/C592S cells; (c) PA/C603S cells; (d) PA/C608S cells; (e) PA/C592S·C603S cells; (f) PA/C592S·C608S cells; (g) PA/C603S·C608S cells; (h) PA/C592S·C603S·C608S cells.