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. 2009 Aug 17;100(12):2283–2291. doi: 10.1111/j.1349-7006.2009.01313.x

Figure 2.

Figure 2

 15‐Lipoxygenase‐1 (15‐LO‐1) reduces the proliferation of colon cancer cells. (A) Two clones of HCT‐116 cells stably expressing 15‐LO‐1 (1E7 and 1F8) were plated in a 96‐well plate and allow to grow for 96 h. The cellular proliferation was determined every 24 h by an MTT assay. The 15‐LO‐1 expressing cells proliferated significantly more slowly (**P < 0.01; ***P < 0.001) compared to empty vector (EV) transfected cells. (B) HT‐29 cells were seeded in 96‐well plates and transiently transfected with the 15‐LO‐1 vector. The proliferation was determined 48 h later by an MTT assay. Significantly slower growth (***P < 0.001) was observed when these cells were transfected with 15‐LO‐1 compared to empty pcDNA3.1 transfected, mock transfected, and parental untransfected HT‐29 cells (HT29_UT). Error bars represent the SD of three independent experiments. ns, not significant.