Figure 2.

 Analysis of the epitopes of P2‐0 and HAG‐3. (a) GST‐tagged recombinant Aggrus deletion mutants were immunoblotted with the indicated antibodies. Schematic representation of the generated Aggrus deletion mutants is shown on upper panel. (b) Synthetic peptides were immobilized on an ELISA plate and incubated with control mouse IgG and P2‐0. Schematic representation of the used synthetic nine amino‐acid peptides is shown in the left panel. Data are means ± standard deviations of triplicate determinations. Control IgG () and P2‐0 (). (c) GST‐tagged recombinant human ΔN20‐Aggrus protein and its point mutants were electrophoresed and immunoblotted with anti‐GST and P2‐0 antibodies.