Figure 3.

 Effects of P2‐0 and HAG‐3 on the Aggrus–C‐type lectin‐like receptor‐2 (CLEC‐2) interaction and platelet aggregation. (a) Recombinant CLEC‐2 protein was immobilized on an ELISA plate and then incubated with the human IgG Fc‐tagged recombinant Aggrus protein in the presence of the indicated concentrations of control mouse IgG1 (control IgG) (), P2‐0 (), and HAG‐3 (). (b) CHO/Aggrus cells were incubated with control mouse IgG1 (control IgG; 10 μg/mL) (), P2‐0) (10 μg/mL [] and 1 μg/mL []) and HAG‐3 (10 μg/mL [] and 1 μg/mL []), followed by incubation with mouse platelets. PBS was added as a negative control (black line). Arrows indicate the time points from the reaction starting point to the half‐maximum value point.