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. 2009 Aug 19;100(12):2309–2317. doi: 10.1111/j.1349-7006.2009.01317.x

Figure 1.

Figure 1

 (a) Microphotographs of three pancreatic cancer cell lines constitutively expressing green fluorescent protein (GFP). (b) The GFP‐SUIT‐2, GFP‐Panc‐1, and GFP‐MIA PaCa‐2 clones used for subsequent experiments were confirmed by flow cytometry to be >99% GFP‐positive in comparison with the non‐GFP‐expressing parental cell lines. (c) GFP fluorescence intensity of GFP‐SUIT‐2 cells. (d) Regression analysis confirmed that the fluorescence intensity of GFP‐SUIT‐2 cells was correlated with the number of cells counted within the range from 5 × 103–1 × 106 cells/well (Spearman’s rank‐correlation coefficient: 0.994, P < 0.0001).