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. 2009 Jun 17;100(10):1801–1808. doi: 10.1111/j.1349-7006.2009.01246.x

Figure 6.

Figure 6

Knockdown DIXDC1 on the proliferative activity of LS174T colon cancer cells. (a) Knockdown of DIXDC1 protein at 60 h after by DIXDC1 siRNA transfection as shown by the immunoblotting analysis. (b) Cells were plated in 96‐well plates at 24 h after DIXDC1 siRNA transfection and detected for OD490 at the indicated time interval with MTT assay. (c) The G1/S cell cycle transition of LS174T cells was inhibited by knockdown of DIXDC1. Columns, mean of three independent experiments; bars, SD. *P < 0.01, compared with control group. (d) LS174T cells were lysed at 60 h after DIXDC1 siRNA transfection. Cell lysates were subjected to immunoblotting with β‐catenin, cyclin D1, p21, Akt, and p‐Akt antibodies as described in ‘Materials and Methods’. Protein levels were normalized to β‐actin.