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. 2009 Nov 18;101(3):735–742. doi: 10.1111/j.1349-7006.2009.01445.x

Figure 1.

Figure 1

 Ad5/3hTERTE1 enhances transgene expression delivered by replication‐ defective adenovirus vectors. (A,B) Control‐combination group SUIT‐2 cells were infected with Ad‐lacZ (25 MOI) and Ad5/3hTERTLuc (0.01 MOI), and CRAd‐combination group SUIT‐2 cells were infected with Ad‐lacZ (25 MOI) and Ad5/3hTERTLuc (0.01 MOI). (A) Total RNA samples were extracted on the indicated days. The expression levels of β‐gal mRNA were measured by qRT‐PCR and normalized by the corresponding expression level of 18S rRNA. Bars represent relative expression levels of mRNA. (B) Photomicrographs of SUIT‐2 cells from the control‐combination and CRAd‐combination groups. β‐Gal activity was assessed by X‐gal staining (magnification, ×100). (C,D) Control‐combination group SUIT‐2 cells were treated with Ad‐NK4 (10 MOI) and Ad5/3hTERTLuc (0.01 MOI or 0.1 MOI), and CRAd‐combination group SUIT‐2 cells were treated with Ad‐NK4 (10 MOI) and Ad5/3hTERTE1 (0.01 MOI or 0.1 MOI). The NK4 expression levels were measured in the culture media (C) and within infected cells (D) on days 1, 2, and 3. Bars represent NK4 concentrations. Each value represents the mean ± SD of three independent samples. **P < 0.01.