Figure 4.

Quantitative real‐time polymerase chain reaction confirmed that mRNA expressions of five genes (a–e) in the interferon‐α (IFNα)‐sensitive line, which are up‐regulated in cDNA microarray analysis, were higher than those in IFNα‐resistant lines (MLLT3 (a), ADFP (b), MTUS1 (c), PME‐1 (d), and MITF (e). In the other cell lines, messenger RNAs (mRNAs) of these five genes are diversely expressed depending on the cell line. Messenger RNA expressions of TNNT1 (f) and MLF1 (g) are concordant with the expression signals detected by cDNA microarray. Correlation of expression of TNNT1 between the IFNα‐sensitive and ‐resistant groups is contrary to the association with ADFP, MTUS1, and MITF. Y‐axis indicates relative mRNA level.