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. 2007 Feb 1;98(3):299–307. doi: 10.1111/j.1349-7006.2007.00403.x

Figure 3.

Figure 3

Effects of NEU4 overexpression on apoptosis induced by TRAIL treatment in human colon cancer cells. At 24 h after transient transfection, HCT‐15 cells were treated with TRAIL plus antipoly histidine for 12 h and apoptotic cells were detected by staining with Hoechst 33258, and counted to give percentages of the total cells (a). Bars indicate the standard deviations of the means for all experiments carried out in triplicate. P‐values were evaluated by the Student's t‐test. Apoptosis was also determined by analyzing cell populations in the subG1 phase fluorometrically (b), and by caspase‐3 activity assay by Western blot with an antibody for cleaved caspase‐3 (c). The NEU4 effect was also observed in the NEU3 silencing cells (d). The sialidase activities towards gangliosides were 4.1, 55.2, 3.4 and 60.8 units/mg protein for mock‐, NEU4‐transfected cells, NEU3 siRNA‐transfected cells, and NEU4‐cDNA and NEU3‐siRNA trans‐fected cells, respectively. The values were the means for two or three experiments in each case.