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Figure 4.

Figure 4

Effects of NEU4 overexpression on apoptosis induced by TRAIL or serum‐depletion in human colon cancer cells. After transient transfection, DLD‐1 cells were treated with TRAIL and apoptotic cells were detected with Hoechst 33258 (a, left panel). DLD‐1 (a, right panel) or HCT‐116 (b) cells were cultured under serum‐depleted conditions for 48 h and apoptotic cells were detected with Hoechst 33258. Bars indicate the standard deviations of the means for experiments carried out in triplicate. P‐values were evaluated by the Student's t‐test.