Figure 5.

Effects of NEU4 overexpression on cell motility and invasion of human colon cancer cells. Twenty‐four hours after transfection, HCT‐15 or DLD‐1 cells were observed for cell motility and invasion using non‐coated and Matrigel‐coated culture inserts, respectively, in the presence of fibronectin in the lower chambers. The cells on the lower surface of the membranes were counted under a microscope. The stable transfectants of DLD‐1 cells, NEU4‐1 and NEU4‐2, showed sialidase activity of 28 and 49 units/mg protein, respectively. Bars indicate the standard deviations of the means for numbers of cells counted in 10 fields. P‐values were evaluated by the Student's t‐test.