Figure 6.

Effects of siRNA‐mediated NEU4 silencing on cell apoptosis, motility and invasion of human colon cancer cells. NEU4 mRNA levels were estimated by real time reverse transcription‐polymerase chain reaction (RT‐PCR) using glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) as the internal control after treatment with NEU4 siRNA or scrambled control (SC) (a). At 24 h after NEU4 siRNA introduction, HCT‐15 cells were treated with TRAIL for 12 h and estimated apoptotic cells (b) and caspase‐3 activity by Western blot (c) as described in Figure 3. Cell migration and invasion were observed using non‐coated and Matrigel‐coated culture inserts in the siRNA‐ or scrambled control‐treated cells (d).