Figure 7.

Thin layer chromatography and Lectin blotting of NEU4 transfected cells. Glycolipids were extracted from NEU4 transfected DLD‐1 cells, subjected to thin layer chromatography and visualized with orcinol‐H₂SO₄ (a). Glycoproteins of the DLD‐1 cells were analyzed by lectin blotting with ricinus communis agglutinin (RCA) or peanut agglutinin (PNA) lectins as described in ‘Materials and Methods’ and compared with those of the mock transfectants (b). Numbers indicate molecular size of the standard markers.