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Tumor cells with enhanced LC3 expression at the peripheral area concomitantly expressed carbonic anhydrase IX (CA IX) as a hypoxia marker. Three photographs of a representative case are shown (hematoxylin–eosin and CA IX and LC3 expression by immununohistochemical staining). The black arrows show the peripheral nerve at the peripheral area of the tumor tissue and which served as a positive internal control of LC3 expression.
