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. 2007 Apr 5;98(6):830–837. doi: 10.1111/j.1349-7006.2007.00459.x

Figure 2.

Figure 2

Effect of anti‐integrin antibodies on endostatin (ED) binding to Lewis lung cancer (LLC) and KLN205 cells. Cells were allowed to adhere for 1 h at 37°C to 96‐well plates coated with ED (20 µg/mL) or bovine serum albumin (BSA; 10 mg/mL) in the absence or presence of anti‐α5 antibody (HMα5), anti‐β1 antibody (HMβ1) or anti‐αv antibody (RMV‐7) at 10 µg/mL. The bound cells were quantitated using the colorimetric WST‐8 agent. Note that HMα5 significantly reduced ED binding to LLC cells (a), but did not influence ED binding to KLN205 cells (b). In contrast, HMβ1 markedly reduced ED binding to KLN205 cells. Data are presented as mean ± SD. *P < 0.01 versus BSA; **P < 0.05 versus coated with ED, soluble (–).