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. 2007 Apr 5;98(6):830–837. doi: 10.1111/j.1349-7006.2007.00459.x

Figure 3.

Figure 3

Cell migration toward endostatin (ED). A cell culture chamber separated by a membrane into an upper and lower chamber was utilized where the membrane surface facing the lower chamber was coated with ED at concentration ranging from 10 to 100 µg/mL or 10 mg/mL bovine serum albumin (BSA). The Lewis lung cancer (LLC) cells were placed in the upper chambers and after 6 h of incubation, cells that migrated through the porous filter were counted at ×400 magnification. (a) LLC cells that migrated toward immobilized ED were observed to a much greater extent than cells that migrated toward immobilized BSA. Data are presented as mean ± SD. *P < 0.001 versus BSA. (b) In contrast to LLC cells, immobilized ED did not induce KLN205 cell migration. (c) Enhanced migration of LLC cells toward ED was abrogated with addition of either ED (50 or 100 µg/mL) or HMα5 (10 µg/mL) to the upper chambers. Data are presented as mean ± SD. *P < 0.0001 versus BSA; **P < 0.0001 versus upper chamber (–), coated with ED 100 µg/mL.