Figure 5.

Effect of i‐Extract on cell motility and Neural Cell Adhesion Molecule (NCAM) expression. i‐Extract reduces C6 glioma cell motility. Representative phase contrast images of control and i‐Extract‐treated cells in the wound‐scratch assay are shown. In the control, the distance between the borderlines of the scratch became significantly narrow 6 h after wounding; it was still wide in i‐Extract‐treated samples. (a) Images shown are at the start point (0 h after scratch) and end point (6 h after scratch) of the assay. (b) NCAM staining in control and i‐Extract‐treated (6 h) cells is shown. There was a significant increase in the NCAM staining in the i‐Extract treatment groups as compared to the control. Western blot shows that the i‐Extract treatment results in increased expression of NCAM 140. (c) Tubulin was used as a loading control.