Figure 4.
Induction and repair of DNA single‐strand breaks (SSB) after ultraviolet (UV) exposure determined using the Comet assay. IB4, SIS, NK‐92 and AIK‐T4 cells were exposed to 10 J/m2 of UV, incubated at 37°C for 0–18 h, then lysed and subjected to Comet assay. (a) The representative pattern of DNA migration (comet tails) from the Comet assay. (b) Quantification of SSB repair assays was carried out as described in Materials and Methods. Compared to control cell lines, IB4 and SIS, NK‐92 and AIK‐T4 cells showed failure and inability to repair DNA SSB after UV exposure. The means (symbol) and standard deviation (error bar) from three experiments is depicted. Symbols represent cell lines: IB4 (□), SIS (○), NK‐92 (•), and AIK‐T4 (▴).