Figure 1.

(A) (–)‐Epigallocatechin‐3‐gallate (EGCG) and imatinib both induce cell death in chronic myelogenous leukemia (CML) cells in a dose‐dependent manner. K562 and C2F8 cells were seeded at a density of 5 × 10³ cells per well in 96‐well plates, and treated with increasing concentrations of EGCG or imatinib for 48 h, and the number of viable cells were measured based on intracellular adenosine triphosphate (ATP) content. Viable cells are expressed as a percentage of 0.1% ethanol‐treated control cells for each cell line. Values are representatives of three independent experiments. (B) EGCG and imatinib showed distinct cell death patterns. K562 cells were treated with 0.1% ethanol (control), EGCG or imatinib for 48 h, harvested and incubated with 3,3′‐dihexyloxacarbocyanine iodide (DiOC₆[3]) and propidium iodide (PI). Mitochondrial transmembrane potential (Δψm) and cell death were determined by dual‐parameter flow cytometry. Results are representative of three independent experiments.