Figure 1.

H₂O₂ or hypoxia followed by recovery/reoxygenation increases Ets1, Ets2, peroxiredoxin1 (Prdx1), and Prdx5 expression. (a,b) PC3 cells were treated with or without 1 mmol/L H₂O₂ for 30 min after which time the media were replaced with fresh media and cells were recovered for the indicated time periods, or hypoxia for 4 h followed by reoxygenation for the indicated time periods. Whole‐cell extracts (100 µg) were subjected to SDS‐PAGE, and Western blotting analysis was performed using the indicated antibodies. Immunoblotting of BAF57 is shown as a loading control. Relative intensity is shown at the bottom of the panel. (c) PC3 cells were treated as described in (a,b). Total RNA (20 µg) was separated on a 1% formaldehyde‐agarose gel and transferred to a Hybond N⁺ membrane. Northern blotting analysis was performed with the indicated cDNA probes. Gel staining with ethidium bromide is also shown.