OD490 values in MCF7 breast cancer cells (a), HeLa uterine cervical cancer cells (b), NUGC‐4 gastric cancer cells (c), B16F0 melanoma cells (d), and MDA‐MB435 melanoma cells (e). The OD490 values of irradiated cell cultures excluding the lowest irradiation group of three shots at 20 J/cm2 decreased significantly (P < 0.001, compared with controls) in all cultures.