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. 2009 Feb 26;100(5):914–919. doi: 10.1111/j.1349-7006.2009.01117.x

Figure 4.

Figure 4

Stanniocalcin 2 (STC2) overexpression promoted cancer cell growth. (a) Western blot analysis with anti‐HA‐tag antibody validated constitutive expression of exogenous STC2 in three stable transformants (Clones 1–3). Mock was the 22Rv1‐mock clone mixture. Coomassie Brilliant Blue (CBB) stain served as a loading control. (b) In vitro growth curve by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide assay showed that three stable transformants (Clones 1–3) grew more rapidly than the 22Rv1‐mock clone mixture (Student's t‐test; *P < 0.01, **P < 0.05). ABS, absorbance at 490 nm, and at 630 nm as a reference, measured with a microplate reader.