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. 2009 Apr 21;100(8):1460–1467. doi: 10.1111/j.1349-7006.2009.01194.x

Figure 6.

Figure 6

Involvement of apoptosis‐inducing factor (AIF) and endonuclease G (EndoG) in cell death by cotreatment with bafilomycin A1 (BMA) and taxol (TXL) in human small cell lung carcinoma Ms‐1 cell lines transfected with Bcl‐xL (Ms‐1/Bcl‐xL). (a) Ms‐1/Bcl‐xL cells were treated BMA (3 nM) and/or TXL (30 ng/mL) for 20 h. AIF and EndoG in the nucleic fraction were immunoblotted with each antibody. Poly(ADP‐ribose) polymerase (PARP) was immunoblotted as a loading control. (b) Ms‐1/Bcl‐xL cells were transfected with siRNA as indicated for 72 h. Cell lysate was immunoblotted with anti‐AIF and anti‐EndoG antibodies. Tubulin was immunoblotted as a loading control. (c) siRNA‐transfected cells were treated with BMA (3 nM) and/or TXL (30 ng/mL) for 36 h. Cell viability was assessed by trypan blue dye exclusion assay. Values are the means of three samples; bars, SD.