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. 2009 Apr 21;100(8):1460–1467. doi: 10.1111/j.1349-7006.2009.01194.x

Figure 7.

Figure 7

Bafilomycin A1 (BMA) promoted translocation to the nucleus of apoptosis‐inducing factor (AIF) and endonuclease G (EndoG) in Ms‐1/neo cells. (a) Ms‐1/neo cells were treated with taxol (TXL) for 20 h. AIF and EndoG in the cytosolic or nucleic fractions were immunoblotted with each antibody. Tubulin and histone H1 were immunoblotted as loading controls. (b) Left, Human small cell lung carcinoma Ms‐1 cell lines transfected with vector control (Ms‐1/neo) were transfected with siRNA as indicated for 72 h. Cell lysate was immunoblotted with anti‐AIF and anti‐EndoG antibodies. Tubulin was immunoblotted as a loading control; right, siRNA‐transfected Ms‐1/neo cells were treated with BMA (3 nM) and/or TXL (30 ng/mL) in the presence or absence of z‐VAD‐fmk (100 µM) for 48 h. Cell viability was assessed by trypan blue dye exclusion assay. Values are the means of three samples; bars, SD.